Cryopreservation of embryos (at the 2-cell stage) and semen is an ideal solution for building up the safety stock of a line in the event of a problem with your breeding program, or to preserve a strain that is no longer (or rarely) used.
Rederivation through fresh embryos or in vitro fertilization (IVF) makes it possible, starting from contaminated animals, to obtain SOPF individuals (free from pathogens and certain opportunistic microorganisms).
Revitalization is used to obtain transgenic mouse lines from frozen embryos or semen that have been stored in cryopreservation straws.
A secure cryogenic room, equipped with two Freezal apparatuses (for slow freezing) and six self-loading tanks for storing cryopreservation straws containing embryos and semen.
